A metabolic trade-off between phosphate and glucose utilization in Escherichia coli

Volker Behrends; Ram P. Maharjan; Ben Ryall; Lu Feng; Bin Liu; Lei Wang; Jacob G. Bundy; Thomas Ferenci

doi:10.1039/c4mb00313f

A metabolic trade-off between phosphate and glucose utilization in Escherichia coli

Volker Behrends, Ram P. Maharjan, Ben Ryall, Lu Feng, Bin Liu, Lei Wang, Jacob G. Bundy, Thomas Ferenci

Research output: Contribution to journal › Article › peer-review

Abstract

Getting the most out of available nutrients is a key challenge that all organisms face. Little is known about how they optimize and balance the simultaneous utilization of multiple elemental resources. We investigated the effects of long-term phosphate limitation on carbon metabolism of the model organism Escherichia coli using chemostat cultures. We profiled metabolic changes in the growth medium over time and found evidence for an increase in fermentative metabolism despite the aerobic conditions. Using full-genome sequencing and competition experiments, we found that fitness under phosphate-limiting conditions was reproducibly increased by a mutation preventing flux through succinate in the tricarboxylic acid cycle. In contrast, these mutations reduced competitive ability under carbon limitation, and thus reveal a conflicting metabolic benefit in the role of the TCA cycle in environments limited by inorganic phosphate and glucose.

Original language	English
Pages (from-to)	2820-2822
Journal	MOLECULAR BIOSYSTEMS
Volume	10
Issue number	11
DOIs	https://doi.org/10.1039/c4mb00313f
Publication status	Published - 2014

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10.1039/c4mb00313f

http://dx.doi.org/10.1039/c4mb00313f

Volker Behrends
- Centre for Integrated Research in Life and Health Sciences
Person

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title = "A metabolic trade-off between phosphate and glucose utilization in Escherichia coli",

abstract = "Getting the most out of available nutrients is a key challenge that all organisms face. Little is known about how they optimize and balance the simultaneous utilization of multiple elemental resources. We investigated the effects of long-term phosphate limitation on carbon metabolism of the model organism Escherichia coli using chemostat cultures. We profiled metabolic changes in the growth medium over time and found evidence for an increase in fermentative metabolism despite the aerobic conditions. Using full-genome sequencing and competition experiments, we found that fitness under phosphate-limiting conditions was reproducibly increased by a mutation preventing flux through succinate in the tricarboxylic acid cycle. In contrast, these mutations reduced competitive ability under carbon limitation, and thus reveal a conflicting metabolic benefit in the role of the TCA cycle in environments limited by inorganic phosphate and glucose.",

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AU - Maharjan, Ram P.

AU - Ryall, Ben

AU - Feng, Lu

AU - Liu, Bin

AU - Wang, Lei

AU - Bundy, Jacob G.

AU - Ferenci, Thomas

PY - 2014

Y1 - 2014

N2 - Getting the most out of available nutrients is a key challenge that all organisms face. Little is known about how they optimize and balance the simultaneous utilization of multiple elemental resources. We investigated the effects of long-term phosphate limitation on carbon metabolism of the model organism Escherichia coli using chemostat cultures. We profiled metabolic changes in the growth medium over time and found evidence for an increase in fermentative metabolism despite the aerobic conditions. Using full-genome sequencing and competition experiments, we found that fitness under phosphate-limiting conditions was reproducibly increased by a mutation preventing flux through succinate in the tricarboxylic acid cycle. In contrast, these mutations reduced competitive ability under carbon limitation, and thus reveal a conflicting metabolic benefit in the role of the TCA cycle in environments limited by inorganic phosphate and glucose.

AB - Getting the most out of available nutrients is a key challenge that all organisms face. Little is known about how they optimize and balance the simultaneous utilization of multiple elemental resources. We investigated the effects of long-term phosphate limitation on carbon metabolism of the model organism Escherichia coli using chemostat cultures. We profiled metabolic changes in the growth medium over time and found evidence for an increase in fermentative metabolism despite the aerobic conditions. Using full-genome sequencing and competition experiments, we found that fitness under phosphate-limiting conditions was reproducibly increased by a mutation preventing flux through succinate in the tricarboxylic acid cycle. In contrast, these mutations reduced competitive ability under carbon limitation, and thus reveal a conflicting metabolic benefit in the role of the TCA cycle in environments limited by inorganic phosphate and glucose.

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DO - 10.1039/c4mb00313f

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EP - 2822

JO - MOLECULAR BIOSYSTEMS

JF - MOLECULAR BIOSYSTEMS

IS - 11

ER -

A metabolic trade-off between phosphate and glucose utilization in Escherichia coli

Abstract

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Volker Behrends

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