A possible role for G-quadruplexes formation and DNA methylation at IMOOD gene promoter in Obsessive Compulsive Disorder

Annalaura Sabatucci; Antonio Girella; Martina Di Bartolomeo; Mariangela Pucci; Matteo Vismara; Beatrice Benatti; Isobel Alice Blacksell; Dianne Cooper; Enrico Dainese; Fulvio D'Acquisto; Bernardo Dell'Osso; Claudio D'Addario

A possible role for G-quadruplexes formation and DNA methylation at IMOOD gene promoter in Obsessive Compulsive Disorder

Annalaura Sabatucci, Antonio Girella, Martina Di Bartolomeo, Mariangela Pucci, Matteo Vismara, Beatrice Benatti, Isobel Alice Blacksell, Dianne Cooper, Enrico Dainese, Fulvio D'Acquisto, Bernardo Dell'Osso, Claudio D'Addario

University of Roehampton

Research output: Contribution to journal › Article › peer-review

Abstract

Obsessive Compulsive Disorder (OCD) is a mental health condition still classified and diagnosed with subjective interview-based assessments and which molecular clues have not completely been elucidated. We have recently identified a new regulator of anxiety and OCD-like behavior called Immuno-moodulin (IMOOD) and, here, we report that IMOOD gene promoter is differentially methylated in OCD subjects when compared to genomic material collected from healthy controls and this alteration is significantly correlated with the increased expression of the gene in OCD. We also demonstrated that IMOOD promoter can form G-quadruplexes and we suggest that, in homeostatic conditions, these structures could evoke DNA-methylation silencing the gene, whereas in pathological conditions, like OCD, could induce gene expression making the promoter more accessible to transcriptional factors. We here thus further suggest IMOOD as a new biomarker for OCD and also hypothesize new mechanisms of gene regulation.

Original language	English
Pages (from-to)	100976
Journal	Advances in biological regulation
Volume	89
Publication status	Published - 8 Aug 2023

Keywords

Humans
DNA Methylation
G-Quadruplexes
Obsessive-Compulsive Disorder/genetics
Gene Expression Regulation
Homeostasis

Access to Document

https://www.sciencedirect.com/science/article/pii/S2212492623000222?via%3Dihub

Cite this

Sabatucci, A., Girella, A., Di Bartolomeo, M., Pucci, M., Vismara, M., Benatti, B., Blacksell, I. A., Cooper, D., Dainese, E., D'Acquisto, F., Dell'Osso, B., & D'Addario, C. (2023). A possible role for G-quadruplexes formation and DNA methylation at IMOOD gene promoter in Obsessive Compulsive Disorder. Advances in biological regulation, 89, 100976. https://www.sciencedirect.com/science/article/pii/S2212492623000222?via%3Dihub

@article{8e9001e446364616a7b353764d316b31,

title = "A possible role for G-quadruplexes formation and DNA methylation at IMOOD gene promoter in Obsessive Compulsive Disorder",

abstract = "Obsessive Compulsive Disorder (OCD) is a mental health condition still classified and diagnosed with subjective interview-based assessments and which molecular clues have not completely been elucidated. We have recently identified a new regulator of anxiety and OCD-like behavior called Immuno-moodulin (IMOOD) and, here, we report that IMOOD gene promoter is differentially methylated in OCD subjects when compared to genomic material collected from healthy controls and this alteration is significantly correlated with the increased expression of the gene in OCD. We also demonstrated that IMOOD promoter can form G-quadruplexes and we suggest that, in homeostatic conditions, these structures could evoke DNA-methylation silencing the gene, whereas in pathological conditions, like OCD, could induce gene expression making the promoter more accessible to transcriptional factors. We here thus further suggest IMOOD as a new biomarker for OCD and also hypothesize new mechanisms of gene regulation.",

keywords = "Humans, DNA Methylation, G-Quadruplexes, Obsessive-Compulsive Disorder/genetics, Gene Expression Regulation, Homeostasis",

author = "Annalaura Sabatucci and Antonio Girella and {Di Bartolomeo}, Martina and Mariangela Pucci and Matteo Vismara and Beatrice Benatti and Blacksell, {Isobel Alice} and Dianne Cooper and Enrico Dainese and Fulvio D'Acquisto and Bernardo Dell'Osso and Claudio D'Addario",

year = "2023",

month = aug,

day = "8",

language = "English",

volume = "89",

pages = "100976",

journal = "Advances in biological regulation",

issn = "2212-4926",

publisher = "Elsevier Ltd.",

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Sabatucci, A, Girella, A, Di Bartolomeo, M, Pucci, M, Vismara, M, Benatti, B, Blacksell, IA, Cooper, D, Dainese, E, D'Acquisto, F, Dell'Osso, B & D'Addario, C 2023, 'A possible role for G-quadruplexes formation and DNA methylation at IMOOD gene promoter in Obsessive Compulsive Disorder', Advances in biological regulation, vol. 89, pp. 100976. <https://www.sciencedirect.com/science/article/pii/S2212492623000222?via%3Dihub>

TY - JOUR

T1 - A possible role for G-quadruplexes formation and DNA methylation at IMOOD gene promoter in Obsessive Compulsive Disorder

AU - Sabatucci, Annalaura

AU - Girella, Antonio

AU - Di Bartolomeo, Martina

AU - Pucci, Mariangela

AU - Vismara, Matteo

AU - Benatti, Beatrice

AU - Blacksell, Isobel Alice

AU - Cooper, Dianne

AU - Dainese, Enrico

AU - D'Acquisto, Fulvio

AU - Dell'Osso, Bernardo

AU - D'Addario, Claudio

PY - 2023/8/8

Y1 - 2023/8/8

N2 - Obsessive Compulsive Disorder (OCD) is a mental health condition still classified and diagnosed with subjective interview-based assessments and which molecular clues have not completely been elucidated. We have recently identified a new regulator of anxiety and OCD-like behavior called Immuno-moodulin (IMOOD) and, here, we report that IMOOD gene promoter is differentially methylated in OCD subjects when compared to genomic material collected from healthy controls and this alteration is significantly correlated with the increased expression of the gene in OCD. We also demonstrated that IMOOD promoter can form G-quadruplexes and we suggest that, in homeostatic conditions, these structures could evoke DNA-methylation silencing the gene, whereas in pathological conditions, like OCD, could induce gene expression making the promoter more accessible to transcriptional factors. We here thus further suggest IMOOD as a new biomarker for OCD and also hypothesize new mechanisms of gene regulation.

AB - Obsessive Compulsive Disorder (OCD) is a mental health condition still classified and diagnosed with subjective interview-based assessments and which molecular clues have not completely been elucidated. We have recently identified a new regulator of anxiety and OCD-like behavior called Immuno-moodulin (IMOOD) and, here, we report that IMOOD gene promoter is differentially methylated in OCD subjects when compared to genomic material collected from healthy controls and this alteration is significantly correlated with the increased expression of the gene in OCD. We also demonstrated that IMOOD promoter can form G-quadruplexes and we suggest that, in homeostatic conditions, these structures could evoke DNA-methylation silencing the gene, whereas in pathological conditions, like OCD, could induce gene expression making the promoter more accessible to transcriptional factors. We here thus further suggest IMOOD as a new biomarker for OCD and also hypothesize new mechanisms of gene regulation.

KW - Humans

KW - DNA Methylation

KW - G-Quadruplexes

KW - Obsessive-Compulsive Disorder/genetics

KW - Gene Expression Regulation

KW - Homeostasis

M3 - Article

C2 - 37572394

SN - 2212-4926

VL - 89

SP - 100976

JO - Advances in biological regulation

JF - Advances in biological regulation

ER -