Direct assessment of metabolite utilization by Pseudomonas aeruginosa during growth on artificial sputum medium

Volker Behrends, Benedikt Geier, Huw D. Williams, Jacob G. Bundy

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We grew Pseudomonas aeruginosa in LB and artificial sputum medium (ASM) (filtered and unfiltered) and quantified metabolite utilization and excretion by nuclear magnetic resonance (NMR) spectroscopy (metabolic footprinting or extracellular metabolomics). Utilization rates were similar between media, but there were differences in excretion-e.g. acetate was produced only in unfiltered ASM.
Original languageEnglish
Pages (from-to)2467-2470
JournalApplied and Environmental Microbiology
Issue number7
Publication statusPublished - 2013

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