Excretion of Avenanthramides, Phenolic Acids and their Major Metabolites Following Intake of Oat Bran

TY - JOUR

T1 - Excretion of Avenanthramides, Phenolic Acids and their Major Metabolites Following Intake of Oat Bran

AU - Schar, Manuel Y

AU - Corona, Giulia

AU - Soycan, Gulten

AU - Dine, Clemence

AU - Kristek, Angelika

AU - Alsharif, Sarah N S

AU - Behrends, Volker

AU - Lovegrove, Alison

AU - Shewry, Peter R.

AU - Spencer, Jeremy P. E.

N1 - This article is protected by copyright. All rights reserved.

PY - 2017/10/11

Y1 - 2017/10/11

N2 - SCOPE: Wholegrain has been associated with reduced chronic disease mortality, with oat intake particularly notable for lowering blood cholesterol and glycemia. To better understand the complex nutrient profile of oats, we studied urinary excretion of phenolic acids and avenanthramides after ingestion of oat bran in humans.METHODS AND RESULTS: After a 2d (poly)phenol-low diet, 7 healthy men provided urine 12h before, and 48h after, consuming 60g oat bran (7.8μmol avenanthramides, 139.2μmol phenolic acids) or a phenolic-low (traces of phenolics) control in a crossover design. Analysis by UPLC-MS/MS showed that oat bran intake resulted in an elevation in urinary excretion of 30 phenolics relative to the control, suggesting that they are oat bran-derived. Mean excretion levels were elevated between 0-2 and 4-8h, following oat bran intake, and amounted to a total of 33.7 ± 7.3μmol total excretion (mean recovery: 22.9 ± 5.0%), relative to control. The predominant metabolites included: vanillic acid, 4- and 3-hydroxyhippuric acids and sulfate-conjugates of benzoic and ferulic acids, which accounted collectively for two thirds of total excretion.CONCLUSION: Oat bran phenolics follow a relatively rapid urinary excretion, with 30 metabolites excreted within 8h of intake. These levels of excretion suggest that bound phenolics are, in part, rapidly released by the microbiota. © 2017, Wiley. The attached document (embargoed until 11/10/2018) is an author produced version of a paper published in Molecular Nutrition & food Research, uploaded in accordance with the publisher’s self- archiving policy. The final published version (version of record) is available online at the link below. Some minor differences between this version and the final published version may remain. We suggest you refer to the final published version should you wish to cite from it.

AB - SCOPE: Wholegrain has been associated with reduced chronic disease mortality, with oat intake particularly notable for lowering blood cholesterol and glycemia. To better understand the complex nutrient profile of oats, we studied urinary excretion of phenolic acids and avenanthramides after ingestion of oat bran in humans.METHODS AND RESULTS: After a 2d (poly)phenol-low diet, 7 healthy men provided urine 12h before, and 48h after, consuming 60g oat bran (7.8μmol avenanthramides, 139.2μmol phenolic acids) or a phenolic-low (traces of phenolics) control in a crossover design. Analysis by UPLC-MS/MS showed that oat bran intake resulted in an elevation in urinary excretion of 30 phenolics relative to the control, suggesting that they are oat bran-derived. Mean excretion levels were elevated between 0-2 and 4-8h, following oat bran intake, and amounted to a total of 33.7 ± 7.3μmol total excretion (mean recovery: 22.9 ± 5.0%), relative to control. The predominant metabolites included: vanillic acid, 4- and 3-hydroxyhippuric acids and sulfate-conjugates of benzoic and ferulic acids, which accounted collectively for two thirds of total excretion.CONCLUSION: Oat bran phenolics follow a relatively rapid urinary excretion, with 30 metabolites excreted within 8h of intake. These levels of excretion suggest that bound phenolics are, in part, rapidly released by the microbiota. © 2017, Wiley. The attached document (embargoed until 11/10/2018) is an author produced version of a paper published in Molecular Nutrition & food Research, uploaded in accordance with the publisher’s self- archiving policy. The final published version (version of record) is available online at the link below. Some minor differences between this version and the final published version may remain. We suggest you refer to the final published version should you wish to cite from it.

U2 - 10.1002/mnfr.201700499

DO - 10.1002/mnfr.201700499

M3 - Article

C2 - 29024323

SN - 1613-4125

JO - Molecular Nutrition and Food Research

JF - Molecular Nutrition and Food Research

ER -