Impact of Gluten-Friendly Bread on the Metabolism and Function of In Vitro Gut Microbiota in Healthy Human and Coeliac Subjects

Antonio Bevilacqua, Adele Costabile, Triana Bergillos-Meca, Isidro Gonzalez, Loretta Landriscina, Emanuela Ciuffreda, Paola D'Agnello, Maria Rosaria Corbo, Milena Sinigaglia, Carmela Lamacchia

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Abstract

The main aim of this paper was to assess the in vitro response of healthy and coeliac human faecal microbiota to gluten-friendly bread (GFB). Thus, GFB and control bread (CB) were fermented with faecal microbiota in pH-controlled batch cultures. The effects on the major groups of microbiota were monitored over 48 h incubations by fluorescence in situ hybridisation. Short-chain fatty acids (SCFAs) were measured by high-performance liquid chromatography (HPLC). Furthermore, the death kinetics of Lactobacillus acidophilus, Bifidobacterium animalis subsp. lactis, Staphylococcus aureus, and Salmonella Typhimurium in a saline solution supplemented with GFB or CB were also assessed. The experiments in saline solution pinpointed that GFB prolonged the survival of L. acidophilus and exerted an antibacterial effect towards S. aureus and S. Typhimurium. Moreover, GFB modulated the intestinal microbiota in vitro, promoting changes in lactobacilli and bifidobacteria members in coeliac subjects. A final multivariate approach combining both viable counts and metabolites suggested that GFB could beneficially modulate the coeliac gut microbiome; however, human studies are needed to prove its efficacy.

Original languageEnglish
Pages (from-to)e0162770
JournalPLoS One
Volume11
Issue number9
DOIs
Publication statusPublished - 15 Sep 2016

Cite this

Bevilacqua, A., Costabile, A., Bergillos-Meca, T., Gonzalez, I., Landriscina, L., Ciuffreda, E., D'Agnello, P., Corbo, M. R., Sinigaglia, M., & Lamacchia, C. (2016). Impact of Gluten-Friendly Bread on the Metabolism and Function of In Vitro Gut Microbiota in Healthy Human and Coeliac Subjects. PLoS One, 11(9), e0162770. https://doi.org/10.1371/journal.pone.0162770