Abstract
We investigated the anti-proliferative effects of an olive oil polyphenolic extract on human colon adenocarcinoma cells. Analysis indicated that the extract contained hydroxytyrosol, tyrosol and the various secoiridoid derivatives, including oleuropein. This extract exerted a strong inhibitory effect on cancer cell proliferation, which was linked to the induction of a G2/M phase cell cycle block. Following treatment with the extract (50 microg/ml) the number of cells in the G2/M phase increased to 51.82+/-2.69% relative to control cells (15.1+/-2.5%). This G2/M block was mediated by the ability of olive oil polyphenols (50 microg/ml) to exert rapid inhibition of p38 (38.7+/-4.7%) and CREB (28.6+/-5.5%) phosphorylation which led to a downstream reduction in COX-2 expression (56.9+/-9.3%). Our data suggest that olive oil polyphenols may exert chemopreventative effects in the large intestine by interacting with signalling pathways responsible for colorectal cancer development.
| Original language | English |
|---|---|
| Pages (from-to) | 606-11 |
| Number of pages | 6 |
| Journal | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS |
| Volume | 362 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 26 Oct 2007 |
Keywords
- Antineoplastic Agents
- Caco-2 Cells
- Cell Cycle
- Cell Line, Tumor
- Cell Proliferation
- Colonic Neoplasms
- Cyclic AMP Response Element-Binding Protein
- Cyclooxygenase 2
- Dose-Response Relationship, Drug
- Flavonoids
- Humans
- MAP Kinase Signaling System
- Olive Oil
- Phenols
- Phosphorylation
- Plant Oils
- Polyphenols
- p38 Mitogen-Activated Protein Kinases