Monoallelic expression of mouse Cd4 gene

Rosanna Capparelli; Adele Costabile; Maurizio Viscardi; Domenico Iannelli

doi:10.1007/s00335-004-2351-y

Monoallelic expression of mouse Cd4 gene

Rosanna Capparelli, Adele Costabile, Maurizio Viscardi, Domenico Iannelli

Research output: Contribution to journal › Article › peer-review

Abstract

A 7-bp deletion in the Cd4 gene, present in the strain MOLF/Ei of Mus musculus molossinus and absent in laboratory mouse strains ( Mus musculus musculus), provided the means to distinguish the parental origin of the Cd4 alleles expressed in single cells of F1 (AKR x MOLF/Ei) and F1 (Balb/C x MOLF/Ei) hybrids. Single-cell RT-PCR showed that the individual CD4+ lymphocyte expresses either the maternal or the paternal Cd4 allele, never both. In situ hybridization proved that Cd4 alleles replicate asynchronously, as expected in the case of genes expressed monoallelically.

Original language	English
Pages (from-to)	579-84
Number of pages	6
Journal	Mammalian genome : official journal of the International Mammalian Genome Society
Volume	15
Issue number	8
DOIs	https://doi.org/10.1007/s00335-004-2351-y
Publication status	Published - Aug 2004

Keywords

Alleles
Animals
Antigens, CD4
Female
Gene Expression
Genomic Imprinting
Male
Mice

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10.1007/s00335-004-2351-y

Cite this

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title = "Monoallelic expression of mouse Cd4 gene",

abstract = "A 7-bp deletion in the Cd4 gene, present in the strain MOLF/Ei of Mus musculus molossinus and absent in laboratory mouse strains ( Mus musculus musculus), provided the means to distinguish the parental origin of the Cd4 alleles expressed in single cells of F1 (AKR x MOLF/Ei) and F1 (Balb/C x MOLF/Ei) hybrids. Single-cell RT-PCR showed that the individual CD4+ lymphocyte expresses either the maternal or the paternal Cd4 allele, never both. In situ hybridization proved that Cd4 alleles replicate asynchronously, as expected in the case of genes expressed monoallelically.",

keywords = "Alleles, Animals, Antigens, CD4, Female, Gene Expression, Genomic Imprinting, Male, Mice",

author = "Rosanna Capparelli and Adele Costabile and Maurizio Viscardi and Domenico Iannelli",

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T1 - Monoallelic expression of mouse Cd4 gene

AU - Capparelli, Rosanna

AU - Costabile, Adele

AU - Viscardi, Maurizio

AU - Iannelli, Domenico

PY - 2004/8

Y1 - 2004/8

N2 - A 7-bp deletion in the Cd4 gene, present in the strain MOLF/Ei of Mus musculus molossinus and absent in laboratory mouse strains ( Mus musculus musculus), provided the means to distinguish the parental origin of the Cd4 alleles expressed in single cells of F1 (AKR x MOLF/Ei) and F1 (Balb/C x MOLF/Ei) hybrids. Single-cell RT-PCR showed that the individual CD4+ lymphocyte expresses either the maternal or the paternal Cd4 allele, never both. In situ hybridization proved that Cd4 alleles replicate asynchronously, as expected in the case of genes expressed monoallelically.

AB - A 7-bp deletion in the Cd4 gene, present in the strain MOLF/Ei of Mus musculus molossinus and absent in laboratory mouse strains ( Mus musculus musculus), provided the means to distinguish the parental origin of the Cd4 alleles expressed in single cells of F1 (AKR x MOLF/Ei) and F1 (Balb/C x MOLF/Ei) hybrids. Single-cell RT-PCR showed that the individual CD4+ lymphocyte expresses either the maternal or the paternal Cd4 allele, never both. In situ hybridization proved that Cd4 alleles replicate asynchronously, as expected in the case of genes expressed monoallelically.

KW - Alleles

KW - Animals

KW - Antigens, CD4

KW - Female

KW - Gene Expression

KW - Genomic Imprinting

KW - Male

KW - Mice

U2 - 10.1007/s00335-004-2351-y

DO - 10.1007/s00335-004-2351-y

M3 - Article

C2 - 15457337

SN - 0938-8990

VL - 15

SP - 579

EP - 584

JO - Mammalian genome : official journal of the International Mammalian Genome Society

JF - Mammalian genome : official journal of the International Mammalian Genome Society

IS - 8

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