Abstract
We investigated the capacity of hydroxytyrosol (HT), 3,4-dihydroxyphenylethanol, and homovanillic alcohol (HVA), 4-hydroxy-3-methoxy-phenylethanol, to inhibit H(2)O(2) induced oxidative damage in LLC-PK1, a porcine kidney epithelial cell line, studying the effect of H(2)O(2) on specific cell membrane lipid targets, unsaturated fatty acids and cholesterol. Exposure to H(2)O(2) induced a significant increase of the level of MDA together with a disruption of the membrane structure, with the loss of unsaturated fatty acids, cholesterol and alpha-tocopherol, and the formation of fatty acids hydroperoxides and 7-ketocholesterol. Pretreatment with HT protected renal cells from oxidative damage: the level of membrane lipids was preserved and there was no significant detection of oxidation products. HVA exerted a comparable activity, thus both HT and HVA were able to prevent in renal cells the lipid peroxidation process that plays a central role in tubular cell injury.
| Original language | English |
|---|---|
| Pages (from-to) | 2984-90 |
| Number of pages | 7 |
| Journal | FOOD AND CHEMICAL TOXICOLOGY |
| Volume | 46 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - Sept 2008 |
Keywords
- Animals
- Antioxidants
- Cholesterol
- Epithelial Cells
- Fatty Acids, Nonesterified
- Homovanillic Acid
- Hydrogen Peroxide
- Kidney Tubules
- LLC-PK1 Cells
- Lipid Peroxidation
- Malondialdehyde
- Membrane Lipids
- Oxidants
- Oxidative Stress
- Phenylethyl Alcohol
- Protective Agents
- Serotonin
- Swine
- alpha-Tocopherol