Protective effect of hydroxytyrosol and its metabolite homovanillic alcohol on H(2)O(2) induced lipid peroxidation in renal tubular epithelial cells

Monica Deiana; Alessandra Incani; Antonella Rosa; Giulia Corona; Angela Atzeri; Debora Loru; M Paola Melis; M Assunta Dessì

doi:10.1016/j.fct.2008.05.037

Protective effect of hydroxytyrosol and its metabolite homovanillic alcohol on H(2)O(2) induced lipid peroxidation in renal tubular epithelial cells

Monica Deiana
, Alessandra Incani
, Antonella Rosa
, Giulia Corona
, Angela Atzeri
, Debora Loru
, M Paola Melis
, M Assunta Dessì

Research output: Contribution to journal › Article › peer-review

Abstract

We investigated the capacity of hydroxytyrosol (HT), 3,4-dihydroxyphenylethanol, and homovanillic alcohol (HVA), 4-hydroxy-3-methoxy-phenylethanol, to inhibit H(2)O(2) induced oxidative damage in LLC-PK1, a porcine kidney epithelial cell line, studying the effect of H(2)O(2) on specific cell membrane lipid targets, unsaturated fatty acids and cholesterol. Exposure to H(2)O(2) induced a significant increase of the level of MDA together with a disruption of the membrane structure, with the loss of unsaturated fatty acids, cholesterol and alpha-tocopherol, and the formation of fatty acids hydroperoxides and 7-ketocholesterol. Pretreatment with HT protected renal cells from oxidative damage: the level of membrane lipids was preserved and there was no significant detection of oxidation products. HVA exerted a comparable activity, thus both HT and HVA were able to prevent in renal cells the lipid peroxidation process that plays a central role in tubular cell injury.

Original language	English
Pages (from-to)	2984-90
Number of pages	7
Journal	FOOD AND CHEMICAL TOXICOLOGY
Volume	46
Issue number	9
DOIs	https://doi.org/10.1016/j.fct.2008.05.037
Publication status	Published - Sept 2008

Keywords

Animals
Antioxidants
Cholesterol
Epithelial Cells
Fatty Acids, Nonesterified
Homovanillic Acid
Hydrogen Peroxide
Kidney Tubules
LLC-PK1 Cells
Lipid Peroxidation
Malondialdehyde
Membrane Lipids
Oxidants
Oxidative Stress
Phenylethyl Alcohol
Protective Agents
Serotonin
Swine
alpha-Tocopherol

Access to Document

10.1016/j.fct.2008.05.037

Cite this

@article{fd24e29bf61142f68d65c51e5af490f1,

title = "Protective effect of hydroxytyrosol and its metabolite homovanillic alcohol on H(2)O(2) induced lipid peroxidation in renal tubular epithelial cells",

abstract = "We investigated the capacity of hydroxytyrosol (HT), 3,4-dihydroxyphenylethanol, and homovanillic alcohol (HVA), 4-hydroxy-3-methoxy-phenylethanol, to inhibit H(2)O(2) induced oxidative damage in LLC-PK1, a porcine kidney epithelial cell line, studying the effect of H(2)O(2) on specific cell membrane lipid targets, unsaturated fatty acids and cholesterol. Exposure to H(2)O(2) induced a significant increase of the level of MDA together with a disruption of the membrane structure, with the loss of unsaturated fatty acids, cholesterol and alpha-tocopherol, and the formation of fatty acids hydroperoxides and 7-ketocholesterol. Pretreatment with HT protected renal cells from oxidative damage: the level of membrane lipids was preserved and there was no significant detection of oxidation products. HVA exerted a comparable activity, thus both HT and HVA were able to prevent in renal cells the lipid peroxidation process that plays a central role in tubular cell injury.",

keywords = "Animals, Antioxidants, Cholesterol, Epithelial Cells, Fatty Acids, Nonesterified, Homovanillic Acid, Hydrogen Peroxide, Kidney Tubules, LLC-PK1 Cells, Lipid Peroxidation, Malondialdehyde, Membrane Lipids, Oxidants, Oxidative Stress, Phenylethyl Alcohol, Protective Agents, Serotonin, Swine, alpha-Tocopherol",

author = "Monica Deiana and Alessandra Incani and Antonella Rosa and Giulia Corona and Angela Atzeri and Debora Loru and \{Paola Melis\}, M and \{Assunta Dess{\`i}\}, M",

year = "2008",

month = sep,

doi = "10.1016/j.fct.2008.05.037",

language = "English",

volume = "46",

pages = "2984--90",

journal = "FOOD AND CHEMICAL TOXICOLOGY",

issn = "0278-6915",

publisher = "Elsevier Ltd.",

number = "9",

}

TY - JOUR

T1 - Protective effect of hydroxytyrosol and its metabolite homovanillic alcohol on H(2)O(2) induced lipid peroxidation in renal tubular epithelial cells

AU - Deiana, Monica

AU - Incani, Alessandra

AU - Rosa, Antonella

AU - Corona, Giulia

AU - Atzeri, Angela

AU - Loru, Debora

AU - Paola Melis, M

AU - Assunta Dessì, M

PY - 2008/9

Y1 - 2008/9

N2 - We investigated the capacity of hydroxytyrosol (HT), 3,4-dihydroxyphenylethanol, and homovanillic alcohol (HVA), 4-hydroxy-3-methoxy-phenylethanol, to inhibit H(2)O(2) induced oxidative damage in LLC-PK1, a porcine kidney epithelial cell line, studying the effect of H(2)O(2) on specific cell membrane lipid targets, unsaturated fatty acids and cholesterol. Exposure to H(2)O(2) induced a significant increase of the level of MDA together with a disruption of the membrane structure, with the loss of unsaturated fatty acids, cholesterol and alpha-tocopherol, and the formation of fatty acids hydroperoxides and 7-ketocholesterol. Pretreatment with HT protected renal cells from oxidative damage: the level of membrane lipids was preserved and there was no significant detection of oxidation products. HVA exerted a comparable activity, thus both HT and HVA were able to prevent in renal cells the lipid peroxidation process that plays a central role in tubular cell injury.

AB - We investigated the capacity of hydroxytyrosol (HT), 3,4-dihydroxyphenylethanol, and homovanillic alcohol (HVA), 4-hydroxy-3-methoxy-phenylethanol, to inhibit H(2)O(2) induced oxidative damage in LLC-PK1, a porcine kidney epithelial cell line, studying the effect of H(2)O(2) on specific cell membrane lipid targets, unsaturated fatty acids and cholesterol. Exposure to H(2)O(2) induced a significant increase of the level of MDA together with a disruption of the membrane structure, with the loss of unsaturated fatty acids, cholesterol and alpha-tocopherol, and the formation of fatty acids hydroperoxides and 7-ketocholesterol. Pretreatment with HT protected renal cells from oxidative damage: the level of membrane lipids was preserved and there was no significant detection of oxidation products. HVA exerted a comparable activity, thus both HT and HVA were able to prevent in renal cells the lipid peroxidation process that plays a central role in tubular cell injury.

KW - Animals

KW - Antioxidants

KW - Cholesterol

KW - Epithelial Cells

KW - Fatty Acids, Nonesterified

KW - Homovanillic Acid

KW - Hydrogen Peroxide

KW - Kidney Tubules

KW - LLC-PK1 Cells

KW - Lipid Peroxidation

KW - Malondialdehyde

KW - Membrane Lipids

KW - Oxidants

KW - Oxidative Stress

KW - Phenylethyl Alcohol

KW - Protective Agents

KW - Serotonin

KW - Swine

KW - alpha-Tocopherol

U2 - 10.1016/j.fct.2008.05.037

DO - 10.1016/j.fct.2008.05.037

M3 - Article

C2 - 18588937

SN - 0278-6915

VL - 46

SP - 2984

EP - 2990

JO - FOOD AND CHEMICAL TOXICOLOGY

JF - FOOD AND CHEMICAL TOXICOLOGY

IS - 9

ER -