Abstract
Bioavailability studies in animals and humans fed with extravirgin olive oil demonstrated that
hydroxytyrosol and tyrosol, the major simple phenolic compounds in extravirgin olive oil, are
dose-dependently absorbed and excreted. Once absorbed, they undergo extensive metabolism;
hydroxytyrosol and tyrosol concentrate mainly in the kidney, where they may exert an important
role in the prevention of oxidative stress induced renal dysfunction. In this study we monitored the
ability of hydroxytyrosol and tyrosol to protect renal cells (LLC-PK1) following oxidative damage
induced by H2O2. Oxidative stress was evaluated by monitoring the changes of the membrane lipid
fraction. Hydroxytyrosol exerted a significant antioxidant action, inhibiting the production of
MDA, fatty acids hydroperoxides and 7-ketocholesterol, major oxidation products of unsaturated
fatty acids and cholesterol, and thus protecting the cells from H2O2-induced damage. Tyrosol,
instead, in this experimental model, did not exert any protective effect.
hydroxytyrosol and tyrosol, the major simple phenolic compounds in extravirgin olive oil, are
dose-dependently absorbed and excreted. Once absorbed, they undergo extensive metabolism;
hydroxytyrosol and tyrosol concentrate mainly in the kidney, where they may exert an important
role in the prevention of oxidative stress induced renal dysfunction. In this study we monitored the
ability of hydroxytyrosol and tyrosol to protect renal cells (LLC-PK1) following oxidative damage
induced by H2O2. Oxidative stress was evaluated by monitoring the changes of the membrane lipid
fraction. Hydroxytyrosol exerted a significant antioxidant action, inhibiting the production of
MDA, fatty acids hydroperoxides and 7-ketocholesterol, major oxidation products of unsaturated
fatty acids and cholesterol, and thus protecting the cells from H2O2-induced damage. Tyrosol,
instead, in this experimental model, did not exert any protective effect.
| Original language | English |
|---|---|
| Pages (from-to) | 301-310 |
| Journal | Toxicology and Industrial Health |
| Volume | 25 |
| Publication status | Published - 2009 |