Protective effect of vanilloids against tert-butyl hydroperoxide-induced oxidative stress in vero cells culture

Antonella Rosa; Angela Atzeri; Monica Deiana; M Paola Melis; Alessandra Incani; Giulia Corona; Debora Loru; Giovanni Appendino; M Assunta Dessì

doi:10.1021/jf073448t

Protective effect of vanilloids against tert-butyl hydroperoxide-induced oxidative stress in vero cells culture

Antonella Rosa, Angela Atzeri, Monica Deiana, M Paola Melis, Alessandra Incani, Giulia Corona, Debora Loru, Giovanni Appendino, M Assunta Dessì

Research output: Contribution to journal › Article › peer-review

Abstract

This study investigated the effect of synthetic capsiate, a simplified analogue of capsiate, and vanillyl alcohol on the oxidative stress induced by tert-butyl hydroperoxide (TBH) in a line of fibroblasts derived from monkey kidney (Vero cells). In response to the TBH-mediated oxidative stress, a reduction of the levels of total unsaturated fatty acids and cholesterol was observed, and a rise in the concentrations of conjugated dienes fatty acids hydroperoxides and 7-ketocholesterol. Pretreatment with both synthetic capsiate and vanillyl alcohol preserved Vero cells from oxidative damage and showed a remarkable protective effect on the reduction of the levels of total unsaturated fatty acids and cholesterol, inhibiting the increase of MDA, conjugated dienes fatty acids hydroperoxides, and 7-ketocholesterol. Both compounds were effective against peroxidation of cell membrane lipids induced by TBH, with synthetic capsiate essentially acting as a pro-drug of vanillyl alcohol, its hydrophilic hydrolytic derivative.

Original language	English
Pages (from-to)	3546-53
Number of pages	8
Journal	Journal of agricultural and food chemistry
Volume	56
Issue number	10
DOIs	https://doi.org/10.1021/jf073448t
Publication status	Published - 28 May 2008

Keywords

Animals
Benzyl Alcohols
Capsaicin
Cell Death
Cercopithecus aethiops
Cholesterol
Fatty Acids, Unsaturated
Fibroblasts
Lipid Peroxidation
Malondialdehyde
Oxidative Stress
Vero Cells
tert-Butylhydroperoxide

Access to Document

10.1021/jf073448t

Cite this

@article{d432f10e024c436995a13671604d32f0,

title = "Protective effect of vanilloids against tert-butyl hydroperoxide-induced oxidative stress in vero cells culture",

abstract = "This study investigated the effect of synthetic capsiate, a simplified analogue of capsiate, and vanillyl alcohol on the oxidative stress induced by tert-butyl hydroperoxide (TBH) in a line of fibroblasts derived from monkey kidney (Vero cells). In response to the TBH-mediated oxidative stress, a reduction of the levels of total unsaturated fatty acids and cholesterol was observed, and a rise in the concentrations of conjugated dienes fatty acids hydroperoxides and 7-ketocholesterol. Pretreatment with both synthetic capsiate and vanillyl alcohol preserved Vero cells from oxidative damage and showed a remarkable protective effect on the reduction of the levels of total unsaturated fatty acids and cholesterol, inhibiting the increase of MDA, conjugated dienes fatty acids hydroperoxides, and 7-ketocholesterol. Both compounds were effective against peroxidation of cell membrane lipids induced by TBH, with synthetic capsiate essentially acting as a pro-drug of vanillyl alcohol, its hydrophilic hydrolytic derivative.",

keywords = "Animals, Benzyl Alcohols, Capsaicin, Cell Death, Cercopithecus aethiops, Cholesterol, Fatty Acids, Unsaturated, Fibroblasts, Lipid Peroxidation, Malondialdehyde, Oxidative Stress, Vero Cells, tert-Butylhydroperoxide",

author = "Antonella Rosa and Angela Atzeri and Monica Deiana and Melis, {M Paola} and Alessandra Incani and Giulia Corona and Debora Loru and Giovanni Appendino and Dess{\`i}, {M Assunta}",

year = "2008",

month = may,

day = "28",

doi = "10.1021/jf073448t",

language = "English",

volume = "56",

pages = "3546--53",

journal = "Journal of agricultural and food chemistry",

issn = "0021-8561",

publisher = "American Chemical Society",

number = "10",

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TY - JOUR

T1 - Protective effect of vanilloids against tert-butyl hydroperoxide-induced oxidative stress in vero cells culture

AU - Rosa, Antonella

AU - Atzeri, Angela

AU - Deiana, Monica

AU - Melis, M Paola

AU - Incani, Alessandra

AU - Corona, Giulia

AU - Loru, Debora

AU - Appendino, Giovanni

AU - Dessì, M Assunta

PY - 2008/5/28

Y1 - 2008/5/28

N2 - This study investigated the effect of synthetic capsiate, a simplified analogue of capsiate, and vanillyl alcohol on the oxidative stress induced by tert-butyl hydroperoxide (TBH) in a line of fibroblasts derived from monkey kidney (Vero cells). In response to the TBH-mediated oxidative stress, a reduction of the levels of total unsaturated fatty acids and cholesterol was observed, and a rise in the concentrations of conjugated dienes fatty acids hydroperoxides and 7-ketocholesterol. Pretreatment with both synthetic capsiate and vanillyl alcohol preserved Vero cells from oxidative damage and showed a remarkable protective effect on the reduction of the levels of total unsaturated fatty acids and cholesterol, inhibiting the increase of MDA, conjugated dienes fatty acids hydroperoxides, and 7-ketocholesterol. Both compounds were effective against peroxidation of cell membrane lipids induced by TBH, with synthetic capsiate essentially acting as a pro-drug of vanillyl alcohol, its hydrophilic hydrolytic derivative.

AB - This study investigated the effect of synthetic capsiate, a simplified analogue of capsiate, and vanillyl alcohol on the oxidative stress induced by tert-butyl hydroperoxide (TBH) in a line of fibroblasts derived from monkey kidney (Vero cells). In response to the TBH-mediated oxidative stress, a reduction of the levels of total unsaturated fatty acids and cholesterol was observed, and a rise in the concentrations of conjugated dienes fatty acids hydroperoxides and 7-ketocholesterol. Pretreatment with both synthetic capsiate and vanillyl alcohol preserved Vero cells from oxidative damage and showed a remarkable protective effect on the reduction of the levels of total unsaturated fatty acids and cholesterol, inhibiting the increase of MDA, conjugated dienes fatty acids hydroperoxides, and 7-ketocholesterol. Both compounds were effective against peroxidation of cell membrane lipids induced by TBH, with synthetic capsiate essentially acting as a pro-drug of vanillyl alcohol, its hydrophilic hydrolytic derivative.

KW - Animals

KW - Benzyl Alcohols

KW - Capsaicin

KW - Cell Death

KW - Cercopithecus aethiops

KW - Cholesterol

KW - Fatty Acids, Unsaturated

KW - Fibroblasts

KW - Lipid Peroxidation

KW - Malondialdehyde

KW - Oxidative Stress

KW - Vero Cells

KW - tert-Butylhydroperoxide

U2 - 10.1021/jf073448t

DO - 10.1021/jf073448t

M3 - Article

C2 - 18439017

SN - 0021-8561

VL - 56

SP - 3546

EP - 3553

JO - Journal of agricultural and food chemistry

JF - Journal of agricultural and food chemistry

IS - 10

ER -