Role of WASP in cell polarity and podosome dynamics of myeloid cells

James Monypenny; Hsiu-Chuan Chou; Inmaculada Bañón-Rodríguez; Adrian J Thrasher; Inés M Antón; Gareth E Jones; Yolanda Calle

doi:10.1016/j.ejcb.2010.05.009

Role of WASP in cell polarity and podosome dynamics of myeloid cells

James Monypenny, Hsiu-Chuan Chou, Inmaculada Bañón-Rodríguez, Adrian J Thrasher, Inés M Antón, Gareth E Jones, Yolanda Calle

Research output: Contribution to journal › Article › peer-review

Abstract

The integrin-dependent migration of myeloid cells requires tight coordination between actin-based cell membrane protrusion and integrin-mediated adhesion to form a stable leading edge. Under this mode of migration, polarised myeloid cells including dendritic cells, macrophages and osteoclasts develop podosomes that sustain the extending leading edge. Podosome integrity and dynamics vary in response to changes in the physical and biochemical properties of the cell environment. In the current article we discuss the role of various factors in initiation and stability of podosomes and the roles of the Wiskott Aldrich Syndrome Protein (WASP) in this process. We discuss recent data indicating that in a cellular context WASP is crucial not only for localised actin polymerisation at the leading edge and in podosome cores but also for coordination of integrin clustering and activation during podosome formation and disassembly.

Original language	English
Pages (from-to)	198-204
Number of pages	7
Journal	European Journal of Cell Biology
Volume	90
Issue number	2-3
DOIs	https://doi.org/10.1016/j.ejcb.2010.05.009
Publication status	Published - 9 Jul 2010

Keywords

Animals
Cell Adhesion
Cell Movement
Cell Polarity
Cytoskeleton
Humans
Mice
Myeloid Cells
Wiskott-Aldrich Syndrome Protein

Access to Document

10.1016/j.ejcb.2010.05.009

Cite this

@article{5470a1da88a043dc9b259070cd81858d,

title = "Role of WASP in cell polarity and podosome dynamics of myeloid cells",

abstract = "The integrin-dependent migration of myeloid cells requires tight coordination between actin-based cell membrane protrusion and integrin-mediated adhesion to form a stable leading edge. Under this mode of migration, polarised myeloid cells including dendritic cells, macrophages and osteoclasts develop podosomes that sustain the extending leading edge. Podosome integrity and dynamics vary in response to changes in the physical and biochemical properties of the cell environment. In the current article we discuss the role of various factors in initiation and stability of podosomes and the roles of the Wiskott Aldrich Syndrome Protein (WASP) in this process. We discuss recent data indicating that in a cellular context WASP is crucial not only for localised actin polymerisation at the leading edge and in podosome cores but also for coordination of integrin clustering and activation during podosome formation and disassembly.",

keywords = "Animals, Cell Adhesion, Cell Movement, Cell Polarity, Cytoskeleton, Humans, Mice, Myeloid Cells, Wiskott-Aldrich Syndrome Protein",

author = "James Monypenny and Hsiu-Chuan Chou and Inmaculada Ba{\~n}{\'o}n-Rodr{\'i}guez and Thrasher, {Adrian J} and Ant{\'o}n, {In{\'e}s M} and Jones, {Gareth E} and Yolanda Calle",

year = "2010",

month = jul,

day = "9",

doi = "10.1016/j.ejcb.2010.05.009",

language = "English",

volume = "90",

pages = "198--204",

journal = "European Journal of Cell Biology",

issn = "0171-9335",

publisher = "Elsevier",

number = "2-3",

}

TY - JOUR

T1 - Role of WASP in cell polarity and podosome dynamics of myeloid cells

AU - Monypenny, James

AU - Chou, Hsiu-Chuan

AU - Bañón-Rodríguez, Inmaculada

AU - Thrasher, Adrian J

AU - Antón, Inés M

AU - Jones, Gareth E

AU - Calle, Yolanda

PY - 2010/7/9

Y1 - 2010/7/9

N2 - The integrin-dependent migration of myeloid cells requires tight coordination between actin-based cell membrane protrusion and integrin-mediated adhesion to form a stable leading edge. Under this mode of migration, polarised myeloid cells including dendritic cells, macrophages and osteoclasts develop podosomes that sustain the extending leading edge. Podosome integrity and dynamics vary in response to changes in the physical and biochemical properties of the cell environment. In the current article we discuss the role of various factors in initiation and stability of podosomes and the roles of the Wiskott Aldrich Syndrome Protein (WASP) in this process. We discuss recent data indicating that in a cellular context WASP is crucial not only for localised actin polymerisation at the leading edge and in podosome cores but also for coordination of integrin clustering and activation during podosome formation and disassembly.

AB - The integrin-dependent migration of myeloid cells requires tight coordination between actin-based cell membrane protrusion and integrin-mediated adhesion to form a stable leading edge. Under this mode of migration, polarised myeloid cells including dendritic cells, macrophages and osteoclasts develop podosomes that sustain the extending leading edge. Podosome integrity and dynamics vary in response to changes in the physical and biochemical properties of the cell environment. In the current article we discuss the role of various factors in initiation and stability of podosomes and the roles of the Wiskott Aldrich Syndrome Protein (WASP) in this process. We discuss recent data indicating that in a cellular context WASP is crucial not only for localised actin polymerisation at the leading edge and in podosome cores but also for coordination of integrin clustering and activation during podosome formation and disassembly.

KW - Animals

KW - Cell Adhesion

KW - Cell Movement

KW - Cell Polarity

KW - Cytoskeleton

KW - Humans

KW - Mice

KW - Myeloid Cells

KW - Wiskott-Aldrich Syndrome Protein

U2 - 10.1016/j.ejcb.2010.05.009

DO - 10.1016/j.ejcb.2010.05.009

M3 - Article

C2 - 20609498

SN - 0171-9335

VL - 90

SP - 198

EP - 204

JO - European Journal of Cell Biology

JF - European Journal of Cell Biology

IS - 2-3

ER -