The MAGUK family protein CARD11 is essential for lymphocyte activation

Hiromitsu Hara; Teiji Wada; Chris Bakal; Ivona Kozieradzki; Shinobu Suzuki; Nobutaka Suzuki; Mai Nghiem; Emily K Griffiths; Connie Krawczyk; Birgit Bauer; Fulvio D'Acquisto; Sankar Ghosh; Wen Chen Yeh; Gottfried Baier; Robert Rottapel; Josef M Penninger

The MAGUK family protein CARD11 is essential for lymphocyte activation

Hiromitsu Hara, Teiji Wada, Chris Bakal, Ivona Kozieradzki, Shinobu Suzuki, Nobutaka Suzuki, Mai Nghiem, Emily K Griffiths, Connie Krawczyk, Birgit Bauer, Fulvio D'Acquisto, Sankar Ghosh, Wen Chen Yeh, Gottfried Baier, Robert Rottapel, Josef M Penninger

Research output: Contribution to journal › Article › peer-review

Abstract

Members of the MAGUK family proteins cluster receptors and intracellular signaling molecules at the neuronal synapse. We report that genetic inactivation of the MAGUK family protein CARD11/Carma1/Bimp3 results in a complete block in T and B cell immunity. CARD11 is essential for antigen receptor- and PKC-mediated proliferation and cytokine production in T and B cells due to a selective defect in JNK and NFkappaB activation. Moreover, B cell proliferation and JNK activation were impaired upon stimulation of TLR4 with lipopolysaccharide, indicating that CARD11 is involved in both the innate and adaptive immune systems. Our results show that the same family of molecules are critical regulators of neuronal synapses and immune receptor signaling.

Original language	English
Pages (from-to)	763-75
Number of pages	13
Journal	Infection and immunity
Volume	18
Issue number	6
Publication status	Published - Jun 2003

Keywords

Animals
Apoptosis Regulatory Proteins
CARD Signaling Adaptor Proteins
Guanylate Cyclase
I-kappa B Proteins
JNK Mitogen-Activated Protein Kinases
Killer Cells, Natural
Lymphocyte Activation
Membrane Glycoproteins
Membrane Proteins
Mice
Mitogen-Activated Protein Kinases
Mutation
Protein Kinase C
Receptors, Antigen
Receptors, Cell Surface
Signal Transduction
Toll-Like Receptor 4
Toll-Like Receptors
Journal Article

Cite this

@article{b7748252201b4898a53a8f6d67d5b32a,

title = "The MAGUK family protein CARD11 is essential for lymphocyte activation",

abstract = "Members of the MAGUK family proteins cluster receptors and intracellular signaling molecules at the neuronal synapse. We report that genetic inactivation of the MAGUK family protein CARD11/Carma1/Bimp3 results in a complete block in T and B cell immunity. CARD11 is essential for antigen receptor- and PKC-mediated proliferation and cytokine production in T and B cells due to a selective defect in JNK and NFkappaB activation. Moreover, B cell proliferation and JNK activation were impaired upon stimulation of TLR4 with lipopolysaccharide, indicating that CARD11 is involved in both the innate and adaptive immune systems. Our results show that the same family of molecules are critical regulators of neuronal synapses and immune receptor signaling.",

keywords = "Animals, Apoptosis Regulatory Proteins, CARD Signaling Adaptor Proteins, Guanylate Cyclase, I-kappa B Proteins, JNK Mitogen-Activated Protein Kinases, Killer Cells, Natural, Lymphocyte Activation, Membrane Glycoproteins, Membrane Proteins, Mice, Mitogen-Activated Protein Kinases, Mutation, Protein Kinase C, Receptors, Antigen, Receptors, Cell Surface, Signal Transduction, Toll-Like Receptor 4, Toll-Like Receptors, Journal Article",

author = "Hiromitsu Hara and Teiji Wada and Chris Bakal and Ivona Kozieradzki and Shinobu Suzuki and Nobutaka Suzuki and Mai Nghiem and Griffiths, {Emily K} and Connie Krawczyk and Birgit Bauer and Fulvio D'Acquisto and Sankar Ghosh and Yeh, {Wen Chen} and Gottfried Baier and Robert Rottapel and Penninger, {Josef M}",

year = "2003",

month = jun,

language = "English",

volume = "18",

pages = "763--75",

journal = "Infection and immunity",

issn = "1074-7613",

publisher = "American Society for Microbiology",

number = "6",

}

TY - JOUR

T1 - The MAGUK family protein CARD11 is essential for lymphocyte activation

AU - Hara, Hiromitsu

AU - Wada, Teiji

AU - Bakal, Chris

AU - Kozieradzki, Ivona

AU - Suzuki, Shinobu

AU - Suzuki, Nobutaka

AU - Nghiem, Mai

AU - Griffiths, Emily K

AU - Krawczyk, Connie

AU - Bauer, Birgit

AU - D'Acquisto, Fulvio

AU - Ghosh, Sankar

AU - Yeh, Wen Chen

AU - Baier, Gottfried

AU - Rottapel, Robert

AU - Penninger, Josef M

PY - 2003/6

Y1 - 2003/6

N2 - Members of the MAGUK family proteins cluster receptors and intracellular signaling molecules at the neuronal synapse. We report that genetic inactivation of the MAGUK family protein CARD11/Carma1/Bimp3 results in a complete block in T and B cell immunity. CARD11 is essential for antigen receptor- and PKC-mediated proliferation and cytokine production in T and B cells due to a selective defect in JNK and NFkappaB activation. Moreover, B cell proliferation and JNK activation were impaired upon stimulation of TLR4 with lipopolysaccharide, indicating that CARD11 is involved in both the innate and adaptive immune systems. Our results show that the same family of molecules are critical regulators of neuronal synapses and immune receptor signaling.

AB - Members of the MAGUK family proteins cluster receptors and intracellular signaling molecules at the neuronal synapse. We report that genetic inactivation of the MAGUK family protein CARD11/Carma1/Bimp3 results in a complete block in T and B cell immunity. CARD11 is essential for antigen receptor- and PKC-mediated proliferation and cytokine production in T and B cells due to a selective defect in JNK and NFkappaB activation. Moreover, B cell proliferation and JNK activation were impaired upon stimulation of TLR4 with lipopolysaccharide, indicating that CARD11 is involved in both the innate and adaptive immune systems. Our results show that the same family of molecules are critical regulators of neuronal synapses and immune receptor signaling.

KW - Animals

KW - Apoptosis Regulatory Proteins

KW - CARD Signaling Adaptor Proteins

KW - Guanylate Cyclase

KW - I-kappa B Proteins

KW - JNK Mitogen-Activated Protein Kinases

KW - Killer Cells, Natural

KW - Lymphocyte Activation

KW - Membrane Glycoproteins

KW - Membrane Proteins

KW - Mice

KW - Mitogen-Activated Protein Kinases

KW - Mutation

KW - Protein Kinase C

KW - Receptors, Antigen

KW - Receptors, Cell Surface

KW - Signal Transduction

KW - Toll-Like Receptor 4

KW - Toll-Like Receptors

KW - Journal Article

M3 - Article

C2 - 12818158

SN - 1074-7613

VL - 18

SP - 763

EP - 775

JO - Infection and immunity

JF - Infection and immunity

IS - 6

ER -